VCF2Dis

VCF2Dis: A new simple and efficient software to calculate p-distance matrix based Variant Call Format

1) Install

The new version will be updated and maintained in hewm2008/VCF2Dis, please click below Link to download the latest version

hewm2008/VCF2Dis

Just [make] or [sh make.sh ] to compile this software.the final software can be found in the Dir [bin/VCF2Dis] For linux /Unix and macOS

        tar -zxvf  VCF2DisXXX.tar.gz            # if Link do not work ,Try re-install [zlib]library
        cd VCF2DisXXX;                          # [zlib] and copy them to the library Dir
        sh make.sh;                             # VCF2Dis-xx/src/include/zlib
        ./bin/VCF2Dis
  

Note: If fail to link,try to re-install the libraries zlib

2) an Example of nj-tree with no boostrap

• 1. Parameter description:

	Usage: VCF2Dis -InPut  <in.vcf>  -OutPut  <p_dis.mat>

		-InPut     <str>     Input one or muti GATK VCF genotype File
		-OutPut    <str>     OutPut Sample p-Distance matrix

		-InList    <str>     Input GATK muti-chr VCF Path List
		-SubPop    <str>     SubGroup SampleList of VCFFile [ALLsample]
		-Rand      <float>   Probability (0-1] for each site to join Calculation [1]
		-KeepMF              Keep the Middle File diff & Use matrix

		-help                Show more help [hewm2008 v1.50]

• 2. To Create the p_distance matrix

# 2.1) To new all the sample p_distance matrix based VCF, run VCF2Dis directly
      ./bin/VCF2Dis	-InPut	in.vcf.gz	-OutPut p_dis.mat
      #  ./bin/VCF2Dis     -InPut  in.fa.gz	-OutPut p_dis.mat -InFormat  FA

# 2.2) To new sub group sample p_distance matrix ; put their sample name into File sample.list
      ./bin/VCF2Dis	-InPut	chr1.vcf.gz chr2.vcf.gz	-OutPut p_dis.mat  -SubPop  sample.list

• 3. construct nj-tree and present it (need deal with Other software)

method 1

Choose one of A/B A. Upload the web fneighbor(http://emboss.toulouse.inra.fr/cgi-bin/emboss/fneighbor?_pref_hide_optional=1) ，the Click the Run fneighbor bottom . then you can get the output file datafile.treefile B. Upload the p_dis.mat to the website fastme (http://www.atgc-montpellier.fr/fastme/), select Data Type to the Distance matrix ,Click the bottom twist execute & email results. you will get the p_dis_mat_fastme-tree.nwk , and Email not mandatory;

Run MEGA # The MEGA (http://www.megasoftware.net/) was used to present the phylogenetic tree based this file [p_dis_mat_fastme-tree.nwk]

method 2

Use the PHYLIPNEW to construct nj-tree How to Install PHYLIPNEW please Click on here or Click on here(Chinese)

      #    3.1 Run  PHYLIP
      #   After p_distance done , software PHYLIPNEW 3.69 (http://evolution.genetics.washington.edu/phylip.html) ,with neighbor-joining method can was used to construct the phylogenetic tree on the basis of this  p_distance matrix;
       
           PHYLIPNEW-3.69.650/bin/fneighbor  -datafile p_dis.matrix  -outfile tree.out1.txt -matrixtype s -treetype n -outtreefile tree.out2.tre

      #    3.2 Run  MEGA
      #    The MEGA6 (http://www.megasoftware.net/) was used to present the phylogenetic tree based this file [tree.out2.tre]

• 4. you can see the neighbor-joining tree and save it as PDF format

3) an Example of nj-tree with boostrap

• 1. muti-run the nj-tree by using put back sampling. To using the the part of the sites and new the nj-tree as above. Repeat For the NN times. X=(1,2....NN);

    ./bin/VCF2Dis	-InPut	in.vcf.gz	-OutPut p_dis_X.mat    -Rand     0.25
    PHYLIPNEW-3.69.650/bin/fneighbor  -datafile p_dis_X.matrix  -outfile tree.out1_X.txt -matrixtype s -treetype n -outtreefile tree.out2_X.tre 

• 2. merge the all the put back sampling NJ-tree and construct boostrap nj-tree.

	cat   tree.out2_*.tre   >  ALLtree_merge.tre
	PHYLIPNEW-3.69.650/bin/fconsense   -intreefile   ALLtree_merge.tre  -outfile out  -treeprint Y
	perl  ./bin/percentageboostrapTree.pl    ALLtree_merge.treefile    NN    Final_boostrap.tre

• 3. construct nj-tree and present it (need deal with Other software)

      #    The MEGA6 (http://www.megasoftware.net/) was used to present the phylogenetic tree based this file Final_boostrap.tre] 

4) Introduction

To new the p_distance matrix besed the VCF file. the more infomation about the p_distance matrix, see this website. The VCF SNPs datasets were used to calculate p-distance between individuals, according to the follow formula to operate the sample i and sample j genetic distance:

            D_ij=(1/L) * [(sum(d(l)_ij))]

Where L is the length of regions where SNPs can be identified, and given the alleles at position l are A/C:

            d(l)_ij=0.0     if the genotypes of the two individuals were AA and AA;
            d(l)_ij=0.5     if the genotypes of the two individuals were AA and AC;
            d(l)_ij=0.0     if the genotypes of the two individuals were AC and AC;
            d(l)_ij=1.0     if the genotypes of the two individuals were AA and CC;
            d(l)_ij=0.0     if the genotypes of the two individuals were CC and CC;

5) Results

some NJ-tree images which I draw in the paper before.

• 50 Rices NBT
• 31 soybeans NG

6) Discussing

• :email: [email protected] / [email protected]
• join the QQ Group : 125293663

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