⛓ Correct misassemblies using linked AND long reads
fastq.gz
and fasta.gz
input read formatssensitive=True
option for ntLink mapping (#113)mapping=ntLink
is added to the tigmint-make
command, use ntLink
for mapping long reads to the draft assembly instead of minimap2
(#110)
memusg
for benchmarking the pipeline (if found in PATH) (#110)tigmint-cut
(#110)btllib
, making it a dependency.fa
, .fa.gz
, .fq
., .fq.gz
SORT_OPTS
parameter for specifying additional parameters for sort
tigmint-make
file to include tigmint steps as targets, such as tigmint-index
(perform the bwa index step)tigmint-make
to include dist
parameter in naming convention of output filesG=0
if unsetG
to be set if using span=auto
in tigmint-make
tigmint_molecule_paf.py
for inferring molecule extents from minimap2 paf mappings (tigmint-long
)tigmint_estimate_dist.py
to estimate the dist
parameter from the first 1M reads (tigmint-long
)long-to-linked-pe
script to C++ (thanks @jowong4)This version of Tigmint includes a couple of changes to the tigmint-long
option:
long-to-linked
produces a fastq file of cut long reads instead of fastadist
parameter is calculated automatically based on the distribution of long read lengths