Estimation of Promoter Activity from RNA-Seq data
Changes in version 1.1.18:
Gene expression data is now stored in the assays of the summarizedExperiment object returned by proActiv to facilitate easier filtering of the summarizedExperiment object. The metadata slot is now empty.
Plotting promoter activity: Implementation of boxplotPromoters function to plot boxplots of absolute promoter activity, relative promoter activity, and gene expression.
Identification of alternative promoters: Implementation of getAlternativePromoters, used to identify promoters that may exhibit alternative usage.
This release corresponds to the Bioconductor 3.12 Release version of proActiv.
This release corresponds to the Bioconductor pre-release version of proActiv. Changes in version 0.99.0:
Workflow: The wrapper function proActiv
performs all steps to estimate promoter activity
and calculates promoter metadata. A condition
argument can be supplied for proActiv
to
summarize promoter counts and activity across conditions. These results are returned as a
SummarizedExperiment object.
BAM file usage: In addition to junction files, proActiv
now allows BAM files as input.
However, users should note that this function is not fully optimized and may have long run-time.
Promoter annotation: Improved efficiency in generating promoter annotations without the need for parallelization with the preparePromoterAnnotations
function. Promoter annotation objects for human (hg19/hg38) and mouse (mm9/mm10) genomes are now pre-calculated and available to the user. The promoter annotation object is also trimmed to preserve essential information for running proActiv
, in order to comply with Bioconductor guidelines concerning package size.
Plotting promoter activity: The plotting function plotPromoters
visualizes promoter activity across conditions. It accepts the SummarizedExperiment object returned by proActiv
along with a gene of interest and gene annotations as arguments. This allows users to visualize promoter activity and identify instances of alternative promoter usage.
Vignette: proActiv now comes with a vignette, documenting a complete step-by-step workflow in identifying active and alternative promoter usage. This includes guidance on running proActiv
, creating promoter annotations and identifying alternative promoter usage. Various visualizations of promoter activity are also offered.
This release corresponds to the proActiv version used by Demircioglu et al.